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Endonuclease IV

Endonuclease IV¡Ê¥¨¥ó¥É¥Ì¥¯¥ì¥¢¡¼¥¼ IV¡Ë¤Ï¡¢DNA¤Î¥¤¥ó¥¿¥¯¥È¤ÊAPÉô°Ì¤ò²Ã¿åʬ²ò¤¹¤ë¥¯¥é¥¹­¶¤Î¥¢¥×¥ê¥ó/¥¢¥Ô¥ê¥ß¥¸¥ó¡ÊAP¡Ë¥¨¥ó¥É¥Ì¥¯¥ì¥¢¡¼¥¼¤Ç¤¹¡£ APÉô°Ì¤Ï»½ý¤Î5 '¦¤Ë¤¢¤ëºÇ½é¤Î¥Û¥¹¥Û¥¸¥¨¥¹¥Æ¥ë·ë¹ç¤ÇÀÚÃǤµ¤ì¡¢3'Ëöü¤Ë¥Ò¥É¥í¥­¥·¥ë´ð¤¬»Ä¤ê¡¢5 'Ëöü¤Ë¥Ç¥ª¥­¥·¥ê¥Ü¡¼¥¹5'-¥ê¥ó»À¤¬»Ä¤ê¤Þ¤¹¡£¤³¤Î¹ÚÁǤÏ3'-¥¸¥¨¥¹¥Æ¥é¡¼¥¼³èÀ­¤â»ý¤Ã¤Æ¤ª¤ê¡¢DNA¤Î3'Ëöü¤«¤é¥Û¥¹¥Û¥°¥ê¥³¥¢¥ë¥Ç¥Ò¥É¡¢¥¤¥ó¥¿¥¯¥È¤Ê¥Ç¥ª¥­¥·¥ê¥Ü¡¼¥¹5-¥ê¥ó»À¤ª¤è¤Ó¥ê¥ó»À¤òÊü½Ð¤¹¤ë¤³¤È¤¬¤Ç¤­¤Þ¤¹

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Fig1.¡¡APÉô°Ì¤ò´Þ¤àDNA¤ÎEndonuclease IV¤Ë¤è¤ëÀÚÃÇ

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10 mM Tris-HCl, 250 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 0.15% Triton X-100, 200 ug/ml BSA, 50% Glycerol, pH 7.4
Reaction buffer (10X)
500 mM Tris-HCl, 1000 mM NaCl, 100 mM MgCl2, 10 mM DTT, pH 7.9
¾¦ÉÊ̾ ÍÆÎÌ Ç»ÅÙ Cat.No.
Endonuclease IV 1,000U 10U/µl M028S
5,000U 10U/µl M028L

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