SurModics社 Stabilization Products

• Stabilization Products
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USE AND SHELF-LIFE QUESTIONS

What are some of the advantages of using SurModics stabilization products?

• Increase shelf life of diagnostic kit components
• Decrease variability often associated with shipping and storage conditions
• Reduce user error by elimination of the conjugate dilution step
• Increase convenience with ready-to-use solutions
• Preservatives used in the StabilZyme products contain no mercury or azide-eliminating the use and disposal restrictions associated with other preservatives
• StabilCoat and StabilGuard stabilize and block in one step

COMPONENT QUESTIONS

What ingredients do the stabilizers contain?

The product formulations are trade secrets. Please refer to the generic description.

How can I get a product approved without knowing what's in it?

• All of our products are manufactured according to QSR guidelines.
• Certificates of Analysis are provided to confirm that the products have met all specifications set for performance prior to being released for sale.
• Material Safety Data Sheets (MSDS) are available for all of the products.
• All of the products are proven, critical components of several commercially available diagnostic devices.

Do the stabilizers contain Thimerosal or Sodium Azide?

No. StabilCoat and StabilGuard do not contain any preservatives. Use aseptic technique in dispensing the reagent or add a preservative compatible with your assay.

The StabilZyme products contain a mercury- and azide-free preservative. The preservative is a mixture of bromonitrodioxane and methylisothiazolone.

Will any of the stabilizers interfere with my assays?

Generally not, but always test first to ensure compatibility with your unique assay. If StabilZyme HRP shows interference in a competitive assay, we recommend you try StabilZyme Select.

StabilCoat and StabilGuard

What is the difference between StabilCoat and StabilGuard?

Both of the products maintain the conformation and biological activity of dried protein components in immunoassays. StabilCoat has a protein component, but StabilGuard contains no animal-source protein, eliminating the possibility of test interference or cross reactivity.

What is the shelf life of the products used for dry stability?

When stored at 4ｰ C, StabilCoat exhibits a 24-month shelf life, and StabilGuard has a shelf life of 36 months.

What shelf life can I expect from an assay component coated with StabilCoat or StabilGuard?

Real-time studies indicate that a shelf life of 30 months at room temperature is achievable. (StabilCoat: 95% for polyclonal and 70% for monoclonal; StabilGuard: 90% for polyclonal and 85% for monoclonal based on an anti-rabbit antibody.)

How should I handle my plates, beads, etc. after stabilizing with StabilCoat or StabilGuard?

Devices must be dried quickly to ensure optimum stabilization. We recommend drying at 20% humidity and using moisture-proof packaging.

Can the stabilizers be diluted?

We recommend that the products be used undiluted for maximum stabilization. However, diluting the products 1:1 will often provide adequate stabilization with significant cost savings. Experiment to see what works best for you.

What can be used to dilute the stabilizers?

StabilCoat---deionized water or PBS

StabilGuard---deionized water or PBS.

The StabilZyme Products

Can I use any of the StabilZyme products for my dried assay components?

No. All of the StabilZyme products are designed to stabilize biologically active components in solution.

What is the difference between StabilZyme HRP and StabilZyme Select?

StabilZyme HRP contains components that increase enzyme activity. While this is beneficial in some assays, it causes interference in others, such as competitive assays. StabilZyme Select was developed to provide stabilization comparable to that of StabilZyme HRP, but without increasing enzyme activity that can interfere with the standard curve in competitive assays. StabilZyme Select is also recommended for direct or sandwich assays where background is a problem.

What is the shelf life of the products used for liquid stability?

When stored at 4ｰ C, all of the StabilZyme products have shelf lives of 30-months. Your conjugate will have an additional shelf life of 24 to 30 months after diluting with one of the StabilZyme products.

What shelf life can I expect from a conjugate added to StabilZyme HRP, StabilZyme AP, or StabilZyme Select?

Real-time studies indicate that a shelf life of 24 months at room temperature is achievable.

• StabilZyme HRP: 90% for anti-rabbit monoclonal-HRP conjugate
• StabilZyme AP: 70% for anti-rabbit monoclonal-AP conjugate
• StabilZyme Select: 75% for anti-rabbit monoclonal-HRP conjugate

Can the StabilZyme stabilizers be diluted?

We recommend that the products be used undiluted for maximum stabilization. However, diluting the products 1:1 will often provide adequate stabilization with significant cost savings. Experiment to see what works best for you.

What can be used to dilute the StabilZyme stabilizers?

• StabilZyme HRP---deionized water.
• StabilZyme AP---deionized water or 10mM MES buffer, pH 6.0.
• StabilZyme Select---deionized water or 0.1 M Tris or Glycine buffer.

NOTE: Phosphate buffers cause precipitation in the StabilZyme products when used at >10% total volume

How much should I dilute my conjugate?

Dilute your conjugated enzyme to the working concentration best suited for your particular assay. For best results, we suggest utilizing a checkerboard assay to determine the optimal dilution for your specific application.

My AP conjugate is extremely unstable. Can I do anything more to increase efficacy?

Try adding 10% glycerol to the StabilZyme AP stabilizer prior to diluting your conjugate.

製品のご購入・ご相談はTEL:03-3545-5720、またはお問い合わせまで。