フォスファターゼ、キナーゼ、ライゲース
Selection Guide for Phosphatases
| 商品名 | APex™ Heat-Labile Alkaline Phospatase | HK™ Phospatase | NTPhos™ Thermolabile Phosphatase | |
| Cat.No. | AP49010(10 反応) AP49050(50 反応) AP49100(100反応) |
H92100(100 MBU) | NT4910K(10,000 U) NT4920K(20,000 U) |
|
| Reaction Temp | 20-50℃、optimal@37℃ | 30℃ | 37℃ | |
| Reaction Time | 10分 | 1時間 | Varies with Substrate Amt | |
| Type of Ends Dephorylated | 5’-Protruding | YES | YES | ― |
| Blunt | YES | NO | ― | |
| 5’-Recessed | YES | NO | ― | |
| Nucleotides are Substrates | YES | NO | YES | |
| Active at pH | 5.5-12 | ~7-9 | ~7-10 | |
| Heat Inactivation | 70℃、5分 | 65℃、15分 | 65℃、15分 | |
Selection Guide for Ligase
| 商品名 | Ampligase® DNA Ligase | CircLigase™ ssDNA Ligase | E.coli DNA Ligase | |
| Lifation Temperature | 20-95℃ | 20-65℃ | 5-20℃ | |
| Cofactor | NAD | ATP | NAD | |
| Ligation Template Required to Ligate |
YES; DNA ONLY |
NO | YES; DNA ONLY |
|
| Type of End Ligated | Blunt | NO | Ligate ssDNA | Weak Activity |
| Cohesive | YES | N/A | YES | |
| Primary Application | Template-Dependent Ligation | Make ssDNA Circles | Make long cDNA | |
| Heat Inactivation | NO; Half-life 95℃、1時間 | 100℃,5分 | 65℃,20分 | |
| 商品名 | Fast-Link™ DNA Ligase | T4 DNA Ligase | T4 RNA Ligase | |
| Lifation Temperature | 5-15分@20℃ | 4-25℃ | 37℃ | |
| Cofactor | ATP | ATP | ATP | |
| Ligation Template Required to Ligate |
NO* | NO* | NO* | |
| Type of End Ligated | Blunt | YES | YES | Ligates ssDNA |
| Cohesive | YES | YES | Ligate RNA to DNA | |
| Primary Application | Rapid Cloning | Cloning | Make chimeric DNA & RNA | |
| Heat Inactivation | 65℃,10分 | 65℃,15分 | 65℃,15分 | |
*These enzymes ligate blunt ends of dsDNA, but ligation is more efficient on a ligation temple, which can be DNA or RNA.
製品のご購入・ご相談はTEL:03-3545-5720、またはお問い合わせまで。
- 10X TA Buffer
| Cat.No. | Quantity |
| TA6115 | 1.5 ml |
| TA6160 | 6.0 ml |
- Enzyme Storage Buffer
| Cat.No. | Quantity |
| ESB4901 | 1 ml |
- Sterile Nuclease-Free Water
| Cat.No. | Quantity |
| W7350ML | 50 ml |
