DNA/RNA Nucleases
DNA and RNA Endonucleases
| Enzyme |
Cat. No. |
Substrate |
Heat Inactive |
| Mung Bean Nuclease |
M8202K(2,000 Units)
M8205K(5,000 Units) |
ssDNA or ssRNA |
No |
- Activity
- Single-strand-specific endonuclease
- Products
- dNMPs or NMPs & oligos with 5’-phosphate & 3’-OH
- Applicatiions
- Converting 5’or 3’overhangs to blunt ends. Removing hairpins after cDNA synthesis. Detecting SNPs by digesting single-base mismatches. With exo III, for making nested deletions.“S1-type”mapping of RNA.
| Enzyme |
Cat. No. |
Substrate |
Heat Inactive |
| OmniCleave™ Endnuclease |
OC7810K(10,000 Units) OC7850K(50,000 Units) |
single- & double- stranded DNA & RNA |
No |
- Activity
- Endonuclease that efficienty digests DNA & RNA.
- Products
- di-, tri-, & tetra- nucleotides
- Applicatiions
- Removal 0f DNA & RNA from protein preps. Removal of host DNA from phage preps.
DNA and RNA Exonuclease
- Activity
- 5’→3’ exonuclease that digests ssDNA or ssRNA with 5’-phosphorylated ends, but not with 5’- hydroxylated ends.
- Products
- dNMPs or NMPs
- Applicatiions
- Removal of 5’-phosphorylated DNA or primers or oligos. Enrichement of ssDNA or ssRNA molecules lacking 5’-phosphate group.
DNA Endonucleases
- Activity
- Activated by divalent cations. In presence of Mg2+, it cleaves each DNA strand randomly &
independently, preferentially adjacent to pyrimidines..
In presence of Mn2+, it cleaves both strands simultaneously, generating fragments with blunt ends or 1
-2-base overhangs.
- Products
- Oligos & dNMPs with 5’-phosphate & 3’-OH.
- Applicatiions
- Removing DNA from RNA preps. Random nicking of dsDNA. DNase footprinting.
| Enzyme |
Cat. No. |
Substrate |
Heat Inactive |
| Endnuclease IV (E. coli) |
E70100(100 Units) |
dsDNA with abasic site |
N/A |
- Activity
- Cleaves sugarphosphate bond 5’ of an abasic site.
- Products
- dsDNA with single-nucleatoide gaps. The cleaved ssDNA strand has a 3’-OH.
- Applicatiions
- DNA repair & anti-tumor drug research. Base Excision Sequence Scanning of DNA containing dumps.
| Enzyme |
Cat. No. |
Substrate |
Heat Inactive |
| T4 Endonuclease V |
TE6605(500 Units) TE661K(1,000 Units) TE665K(5,000 Units) |
UV-Irradiated DNA
with Thymine Dimers |
N/A |
- Activity
- First cleaves N-glycosidic bond 5’ of thymine dimmers, then cleaves sugar-phosphate bond 3’ of the abasic site.
- Products
- Nicked dsDNA with an abasic site at the 3’-end of the cut & thymine-dimer bases at the 5’-end of the cut
- Applicatiions
- Reseach on repair of DNA exposed to UV light.
| Enzyme |
Cat. No. |
Substrate |
Heat Inactive |
| Lambda Terminase |
LT4450(50 Units) LT44200(200 Units) |
dsDNA with cos sites |
N/A |
- Activity
- Cleaves both strands at bacteriophage lambda cos sites.
- Products
- 5’-ends with over-hangs 12 bases in length.
- Applicatiions
- Rapid sizing or Restriction mapping of BAC, fosmid or cosmid clones.
DNA Exonucleases
| Enzyme |
Cat. No. |
Substrate |
Heat Inactive |
| Exonuclease I (E. coli) |
X40501K(1,000 Units) X40505K(5,000 Units) X40520K(20,000 Units) |
ssDNA |
80℃ for 15 min |
- Activity
- 3’→5’exonuclease
- Products
- DNMPs
- Applicatiions
- Removal of ssDNA & oligonucletides
| Enzyme |
Cat. No. |
Substrate |
Heat Inactive |
| Exonuclease III (E. coli) |
EX4405K(5,000 Units) EX4425K(25,000 Units) |
dsDNA |
70℃ for 20 min |
- Activity
- 3’→5’exonuclease that digests duplex DNA from the 3’-end of a nick or a blunt or 3’-recessed end; not active on thionucleotides. Exo III also has RNase H, 3’-DNA phosphatase & apurinic DNA endonuclease activities.
- Products
- DNMPs & ssDNA on the opposite strand. Partial digestion produces dsDNA having 5’extensions of ssDNA.
- Applicatiions
- Used with S1 Nuclease or Mung Bean Nuclease to make nested deletions. Preparation of ssDNA templates for sequencing. Site-directed mutagenesis. Preparation of labeled strand-specific probes
| Enzyme |
Cat. No. |
Substrate |
Heat Inactive |
| Exonuclease VII |
EN510100(100 Units) EN510250(250 Units) |
ssDNA |
95℃ for 10 min |
- Activity
- Exonuclease that digests in both 5’→3’& 3’→5’directions.
- Products
- dNMPs
- Applicatiions
- Removal of primers & single- stranded oligos.
| Enzyme |
Cat. No. |
Substrate |
Heat Inactive |
| Lambda Exonuclease |
LE035H(500 Units) LE032K(2,500 Units) |
dsDNA |
75℃ for 10 min |
- Activity
- 5’→3’exonuclease that digests dsDNA from 5’-phosphorylated blunt or recessed ends. It has low activity on 5’-hydroxylated ends & is not active on nicked DNA.
- Products
- dNMPs & ssDNA-on the opposite strand. Partial digestion Produces dsDNA having 3’ extensions of ssDNA
- Applicatiions
- Preparation of ssDNA templates for sequencing.
| Enzyme |
Cat. No. |
Substrate |
Heat Inactive |
| RecBCD Nuclease (E. coli) |
BCD0401K(1,000 Units) |
dsDNA & ssDNA |
N/A |
- Activity
- An ATP- & Mg2+- dependent exonuclease that digests linear DNA in both 5’→3’ & 3’→5’
directions. Not active on nicked or closed-circular dsDNA.
- Products
- dNMPs
- Applicatiions
- Removal of linear DNA from circular DNA.
| Enzyme |
Cat. No. |
Substrate |
Heat Inactive |
| Rec J Exonuclease |
RJ411050(50 Units)
RJ411250(250 Units) |
ssDNA |
65℃ for 20 min |
- Activity
- 5’→3’exonuclease that digests ssDNA with a 5’-phosphate or a 5’-OH.
- Products
- dNMPs
- Applicatiions
- Removal of primer & ssDNA from dsDNA.
| Enzyme |
Cat. No. |
Substrate |
Heat Inactive |
| T5 Exonuclease |
T5E4111K(1,000 Units) |
dsDNA & ssDNA |
N/A |
- Activity
- 5’→3’exonuclease that also has single-strand-specific endonuclease activity in presence of 1-10 mM
Mg2+ ions. At ＜1 mM Mg2+, the 5’→3’exonuclease can digest from a nick in closed-circular
dsDNA without digesting the opposite strand.
- Products
- dNMPs. Circular ssDNA is Obtained from Closed-circular dsDNA in presence of ＜1 mM Mg2+.
- Applicatiions
- Plasmid mutagenesis. Oligonucleotide site-directed mutagenesis. Removing linear DNA from plasmid
preps. Preparation of circular ssDNA.
RNA Endonucleases
| Enzyme |
Cat. No. |
Substrate |
Heat Inactive |
| RNase A |
MRNA092(2 ml @ 5 mg/ml) |
ssRNA |
N/A |
- Activity
- Cleaves ssRNA 3’of pyrimidine residues.
- Products
- Oligoribonucleotides with 3’-cytidine or 3’-uridine residues
- Applicatiions
- Removal of RNA from DNA preps. RNase protection assays. RNA mapping & structure studies.
| Enzyme |
Cat. No. |
Substrate |
Heat Inactive |
| RNase I, E. coli |
N6901K(1,000 Units) N6905K(5,000 Units) |
ssRNA |
70℃ for 15 min |
- Activity
- Cleaves ssRNA between all dinucleotide pairs.
- Products
- NMPs with 5’-OH & 2’, 3’-cyclic monophosphate
- Applicatiions
- Removal of RNA from DNA preps. RNase protection assays. Mismatch detection of single basepairs in RNA:RNA or RNA:DNA hybrids.
| Enzyme |
Cat. No. |
Substrate |
Heat Inactive |
| RNase III, E. coli |
RN02950(50 Units) |
dsRNA |
No
5’-phosphate |
- Activity
- Cleaves dsRNA in presence of Mg2+ to 12-15-bp dsRNA. Cleaves dsRNA in presence of 20 mM
Co2+ or Mn2+ to 18-25-bp dsRNA.
- Products
- dsRNA with 5’-phosphate & 2-base 3’-overhangs with 3’-OH
- Applicatiions
- Random cleavage of long dsRNA to short dsRNA. RNA interference (RNAi). Studies on RNA structure, RNA processing & maturation.
| Enzyme |
Cat. No. |
Substrate |
Heat Inactive |
| RNase H, E. coli |
R52250(250 Units) R0601K(1,000 Units) |
RNA in RNA:DNA Hybrid |
65℃ for 10 min |
- Activity
- Cleaves RNA in RNA:DNA hybrid without affecting unhybridized RNA or DNA
- Products
- oligoribonucleotides with 5’-phosphate & 3’-OH
- Applicatiions
- Elimination of RNA prior to second strand cDNA synthesis. Removal of poly(A) tails from mRNA hybridized to oligo(dT).
| Enzyme |
Cat. No. |
Substrate |
Heat Inactive |
| Hybridase™ Thermostable RNase H |
H39100(100 Units) H39500(500 Units) |
RNA in RNA:DNA Hybrid |
No |
- Activity
- Cleaves RNA in RNA:DNA Hybrid without affecting Unhybridzed RNA or DNA.
- Products
- oligoribonucleotides with 5’-phosphate & 3’-OH
- Applicatiions
- High-stringency hybrid selection. High-stringency mapping of mRNA structure. Transcription-based amplification methods.
| Enzyme |
Cat. No. |
Substrate |
Heat Inactive |
| RNase T1, Aspergillus oryzae |
NT09100K(100,000 Units) NT09500K(500,000 Units) |
ssRNA |
N/A |
- Activity
- Cleaves ssRNA 3’of GMPs.
- Products
- oligoribonucleotides with 3’-GMP residues
- Applicatiions
- RNA mapping & structure studies. Removal of RNA from DNA preps.
| Enzyme |
Cat. No. |
Substrate |
Heat Inactive |
| RiboShredder™ RNase Blend |
RS12100(100 Units) RS12500(500 Units) |
ssRNA |
No |
- Activity
- Efficiently degrades all RNA.
- Products
- NMPs
- Applicatiions
- Removal of all RNA from genomic & cloned DNA preps.
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