DNA Polymerases
Mesophilic DNA Polymerases
DNA Polymerase I, E. coli
DP02250 (250 U), DP021K (1,000 U), DP0415K (5,000 U)
| 5’→3’ exonuclease |
3’→5’ exonuclease |
Nick translation | Heat Inactivationa |
Strand displacement |
| ＋ | ＋＋ | ＋ | 75℃, 20分 | − |
Klenow DNA Polymerase
KP04061K (1,000 U)
| 5’→3’ exonuclease |
3’→5’ exonuclease |
Nick translation | Heat Inactivationa |
Strand displacement |
| − | ＋＋ | − | 75℃, 20分 | ＋ |
Exo-Minus Klenow DNA Polymerase
KL04011K (1,000 U)
| 5’→3’ exonuclease |
3’→5’ exonuclease |
Nick translation | Heat Inactivationa |
Strand displacement |
| − | − | − | 75℃, 20分 | ＋ |
RepliPHI™ Phi29 DNA Polymerase
| 5’→3’ exonuclease |
3’→5’ exonuclease |
Nick translation | Heat Inactivationa |
Strand displacement |
| − | ＋＋ | − | 65℃, 10分 | ＋＋＋＋ |
T4 DNA Polymerase
D0602H (200 U), D0605H (500 U)
| 5’→3’ exonuclease |
3’→5’ exonuclease |
Nick translation | Heat Inactivationa |
Strand displacement |
| − | ＋＋＋ | − | 75℃, 20分 | − |
a Indicated treatment results in complete inactivation under standard reaction conditions.
Thermophilic DNA Polymerases
rBst DNA Polymeraseb
| Reverse transcriptase |
5’→3’ exonuclease |
3’→5’ exonuclease |
Thermostabilityc | Fidelityd | Strand displacement |
| ＋ | ＋ | − | <15分, 75℃ <1.5分, 95℃ |
N/A | − |
rBst DNA Polymerase, Large Fragment (IsoTherm™ DNA Polymerase)
| Reverse transcriptase |
5’→3’ exonuclease |
3’→5’ exonuclease |
Thermostabilityc | Fidelityd | Strand displacement |
| ＋ | − | − | <15分, 75℃ <1.5分, 95℃ |
N/A | ＋＋＋ |
MasterAmp™ AmpliTherm™ DNA Polymerase
| Reverse transcriptase |
5’→3’ exonuclease |
3’→5’ exonuclease |
Thermostabilityc | Fidelityd | Strand displacement |
| − | − | − | N/A | N/A | − |
MasterAmp™ Taq DNA Polymerase
| Reverse transcriptase |
5’→3’ exonuclease |
3’→5’ exonuclease |
Thermostabilityc | Fidelityd | Strand displacement |
| Very Week Requires Mn2+ | ＋ | − | 97.5℃, 9分 | 0.38-1.82×104 | − |
MasterAmp™ Tfl DNA Polymerase
| Reverse transcriptase |
5’→3’ exonuclease |
3’→5’ exonuclease |
Thermostabilityc | Fidelityd | Strand displacement |
| − | ＋ | − | 95℃, 40分 | 8.3-9.0×105 | − |
MasterAmp™ Tth DNA Polymerase
| Reverse transcriptase |
5’→3’ exonuclease |
3’→5’ exonuclease |
Thermostabilityc | Fidelityd | Strand displacement |
| ＋＋ Requires Mn2+ |
＋ | − | 95℃, 20分 | 2.2×104 | − |
b rBst DNA polymerase and rBst DNA Large Fragment (IsoTherm™ DNA Polymerase) are for DNA replication at <65℃. Not suitable for typical PCR. The other themostable enzymes listed in this chart are suitable for PCR cycling applications.
c Values represent half-lives: 50% of the enzymatic activity is retained after the given time at the temperature.
d Defined as the average number of correct nucleotides a polymerase incorporates before making an error.
N/A - Not available.
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