製品案内

  • アプリケーションノート
  • キャンペーン情報はこちら
  • デモ機サンプルはこちら
  • 製品動画はこちら
  • 03-3545-5720(東京)
  • 06-6282-4004(大阪)
お問い合わせはこちら

End-It™ DNA End-Repair Kit

End-It™ DNA End-Repair Kitは、5’、もしくは3'の突出末端DNAを5'-phosphorylationします。blunt-ended DNAは、プラスミドやコスミド、フォスミド、BACなどへのblunt-end cloningに使用できます。blunt-ended DNAへの転換は5'→3' polymeraseによる伸長とT4 DNA Polymeraseの3'→5' exonuclease活性により行われます。blunt-ended DNAのphosphorylation のためのT4 Polynucleotide KinaseとATPもキットに含まれます。End-It DNA End-Repair KitはEPICENTRE社pWEB™ Cosmid Cloning Kit、pWEB-TNC™ Cosmid Cloning Kit、EpiFOS™ Fosmid Library Production Kit、 CopyControl™ Fosmid Library Production Kitで提供されているEnd-Repair Enzyme Mixと同じものです。

End-It DNA End-Repair Kitは20反応分(repair of up to 100 μg of genomic DNA)の試薬が含まれています。end-repaired, 5'-phosphorylated DNAは、Fast-Link DNA Ligation Kitを使用することでblunt-ended, dephosphorylated cloning vectorへ効率的にライゲーションすることが可能です。

Application

Prepare sheared, nebulized, or restriction enzyme digested genomic DNA for blunt-end cloning into plasmid, cosmid, fosmid, or BAC vectors.

Benefits

The repaired DNA is blunt-ended and 5'-phosphorylated for immediate blunt-end ligation into plasmid, cosmid, fosmid, or BAC vectors.

High specific activity T4 DNA Polymerase and T4 Polynucleotide Kinase are included for complete conversion of 5'- or 3'-overhang DNA to 5'-phosphorylated, blunt-ended DNA.

Figure 1

Figure 1. DNA fragments containing any type of ends are rapidly and efficiently converted to 5'-phosphorylated, blunt-ended DNA using the End-It™ DNA End-Repair Kit.

The end-repaired DNA is ready for blunt-end ligation into the cloning vector of choice using, for example EPICENTRE's Fast-Link™ DNA Ligation Kit.

商品名 Cat.No. Size
End-It™ DNA End-Repair Kit ER0720 20 Reactions
ER81050 50 Reactions

Kit Contents

  • End-Repair Enzyme Mix including : T4 DNA Polymerase and T4 Polynucleotide Kinase
  • End-Repair 10X Buffer
  • dNTP Solution
  • ATP

PAGE TOP